Optimized Ultraviolet-C Processing Inactivates Pathogenic and Spoilage-Associated Bacteria while Preserving Bioactive Proteins, Vitamins, and Lipids in Human Milk

2024  Journal Article

Optimized Ultraviolet-C Processing Inactivates Pathogenic and Spoilage-Associated Bacteria while Preserving Bioactive Proteins, Vitamins, and Lipids in Human Milk

Pub TLDR

UV-C treatment is a promising way to process donor milk that balances safety with preserving the beneficial components that support the health of vulnerable babies. Using UV-C in milk banks could help provide higher quality donor milk to premature or sick infants who depend on it for protection against infection and optimal development.

DOI: 10.1021/acs.jafc.4c02120    PubMed ID: 38752986
 

College of Health researcher(s)

Abstract

Holder pasteurization (HoP) enhances donor human milk microbiological safety but damages many bioactive milk proteins. Though ultraviolet-C irradiation (UV–C) can enhance safety while better preserving some milk proteins, it has not been optimized for dose or effect on a larger array of bioactive proteins. We determined the minimal UV–C parameters that provide >5-log reductions of relevant bacteria in human milk and how these treatments affect an array of bioactive proteins, vitamin E, and lipid oxidation. Treatment at 6000 and 12 000 J/L of UV–C resulted in >5-log reductions of all vegetative bacteria and bacterial spores, respectively. Both dosages improved retention of immunoglobulin A (IgA), IgG, IgM, lactoferrin, cathepsin D, and elastase and activities of bile-salt-stimulated lipase and lysozyme compared with HoP. These UV–C doses caused minor reductions in α-tocopherol but not γ-tocopherol and no increases in lipid oxidation products. UV–C treatment is a promising approach for donor human milk processing.

Liang, N., Mohamed, H., Pung, R.F., Waite-Cusic, J., Dallas, D.C. (2024) Optimized Ultraviolet-C Processing Inactivates Pathogenic and Spoilage-Associated Bacteria while Preserving Bioactive Proteins, Vitamins, and Lipids in Human MilkJournal of Agricultural and Food Chemistry