Title | Vitamin E Deficiency Disrupts Gene Expression Networks during Zebrafish Development. |
Publication Type | Journal Article |
Year of Publication | 2021 |
Authors | Head, B, Ramsey, SA, Kioussi, C, Tanguay, RL, Traber, M |
Journal | Nutrients |
Volume | 13 |
Issue | 2 |
Date Published | 01/2021 |
ISSN | 2072-6643 |
Abstract | Vitamin E (VitE) is essential for vertebrate embryogenesis, but the mechanisms involved remain unknown. To study embryonic development, we fed zebrafish adults (>55 days) either VitE sufficient (E+) or deficient (E-) diets for >80 days, then the fish were spawned to generate E+ and E- embryos. To evaluate the transcriptional basis of the metabolic and phenotypic outcomes, E+ and E- embryos at 12, 18 and 24 h post-fertilization (hpf) were subjected to gene expression profiling by RNASeq. Hierarchical clustering, over-representation analyses and gene set enrichment analyses were performed with differentially expressed genes. E- embryos experienced overall disruption to gene expression associated with gene transcription, carbohydrate and energy metabolism, intracellular signaling and the formation of embryonic structures. mTOR was apparently a major controller of these changes. Thus, embryonic VitE deficiency results in genetic and transcriptional dysregulation as early as 12 hpf, leading to metabolic dysfunction and ultimately lethal outcomes. |
DOI | 10.3390/nu13020468 |
Alternate Journal | Nutrients |
PubMed ID | 33573233 |
PubMed Central ID | PMC7912379 |