TitleTissue specificity of S14 and fatty acid synthase in vitro transcription.
Publication TypeJournal Article
Year of Publication1992
AuthorsMacDougald, OA, Clarke, SD, Jump, DB
JournalBiochem Biophys Res Commun
Volume182
Issue2
Pagination631-7
Date Published1992 Jan 31
ISSN0006-291X
KeywordsAnimals, Cell Nucleus, Fatty Acid Synthases, Gene Library, Kidney, Kinetics, Liver, Nuclear Proteins, Organ Specificity, Promoter Regions, Genetic, Proteins, Rats, Spleen, Transcription Factors, Transcription, Genetic
Abstract

Hepatic nuclear extracts support in vitro transcription from S14 and fatty acid synthase (FAS) promoters. Renal nuclear extracts support in vitro transcription from S14 promoter constructs at rates comparable to hepatic nuclear extracts, but lack the apparent positive and negative control observed with liver. In contrast, renal nuclear extracts do not support in vitro transcription from the FAS promoter. NF-1 or a related protein in both hepatic and renal extracts contributes to in vitro transcriptional activity from the S14 promoter, but not the FAS promoter. These studies indicate that different mechanisms regulate tissue-specific expression of S14 and FAS.
Alternate JournalBiochem. Biophys. Res. Commun.
PubMed ID1734874
Grant ListDK43220 / DK / NIDDK NIH HHS / United States