TitleThe role of gut microbiota in fetal methylmercury exposure: Insights from a pilot study.
Publication TypeJournal Article
Year of Publication2016
AuthorsRothenberg, SE, Keiser, S, Ajami, NJ, Wong, MC, Gesell, J, Petrosino, JF, Johs, A
JournalToxicol Lett
Volume242
Pagination60-67
Date Published03/2016
ISSN1879-3169
KeywordsBacteria, Bacterial Proteins, Biotransformation, Feces, Female, Fetal Blood, Gastrointestinal Microbiome, Gastrointestinal Tract, Gene Expression Profiling, Gestational Age, Hair, Humans, Lyases, Maternal Exposure, Maternal-Fetal Exchange, Metagenome, Metagenomics, Methylmercury Compounds, Oxidoreductases, Pilot Projects, Pregnancy, Pregnancy Trimester, Third, Ribotyping
Abstract
 

PURPOSE: The mechanisms by which gut microbiota contribute to methylmercury metabolism remain unclear. Among a cohort of pregnant mothers, the objectives of our pilot study were to determine (1) associations between gut microbiota and mercury concentrations in biomarkers (stool, hair and cord blood) and (2) the contributions of gut microbial mercury methylation/demethylation to stool methylmercury.

METHODS: Pregnant women (36-39 weeks gestation, n=17) donated hair and stool specimens, and cord blood was collected for a subset (n=7). The diversity of gut microbiota was determined using 16S rRNA gene profiling (n=17). For 6 stool samples with highest/lowest methylmercury concentrations, metagenomic whole genome shotgun sequencing was employed to search for the mercury methylation gene (hgcA), and two mer operon genes involved in methylmercury detoxification (merA and merB).

RESULTS: Seventeen bacterial genera were significantly correlated (increasing or decreasing) with stool methylmercury, stool inorganic mercury, or hair total mercury; however, aside from one genus, there was no overlap between biomarkers. There were no definitive matches for hgcA or merB, while merA was detected at low concentrations in all six samples.

MAJOR CONCLUSIONS: Proportional differences in stool methylmercury were not likely attributed to gut microbiota through methylation/demethylation. Gut microbiota potentially altered methylmercury metabolism using indirect pathways.

DOI10.1016/j.toxlet.2015.11.022
Alternate JournalToxicol. Lett.
PubMed ID26626101
PubMed Central IDPMC4707065
Grant ListL30 ES023165 / ES / NIEHS NIH HHS / United States
R15 ES022409 / ES / NIEHS NIH HHS / United States