TitleRegulation of the alpha-tocopherol transfer protein in mice: lack of response to dietary vitamin E or oxidative stress.
Publication TypeJournal Article
Year of Publication2006
AuthorsBella, DL, Schock, BC, Lim, Y, Leonard, SW, Berry, C, Cross, CE, Traber, M
Date Published2006 Feb
KeywordsAnimals, Body Weight, Carrier Proteins, Diet, Eating, Fasting, Gene Expression Regulation, Liver, Male, Mice, Mice, Inbred C57BL, Oxidative Stress, Tobacco Smoke Pollution, Vitamin E

The alpha-tocopherol transfer protein (TTP) plays an important role in the regulation of plasma alpha-tocopherol concentrations. We hypothesized that hepatic TTP levels would be modulated by dietary vitamin E supplementation and/or by oxidative stress. Mice were fed either a High E (1150 mg RRR-alpha-tocopheryl acetate/kg diet) or a Low E (11.5 mg/kg diet) diet for 2 wk. High E increased plasma and liver alpha-tocopherol concentrations approximately 8- and 40-fold, respectively, compared with Low E-fed mice, whereas hepatic TTP increased approximately 20%. Hepatic TTP concentrations were unaffected by fasting (24 h) in mice fed either diet. To induce oxidative stress, chow-fed mice were exposed for 3 d to environmental tobacco smoke (ETS) for 6 h/d (total suspended particulate, 57.4 +/- 1.8 mg/m3). ETS exposure, while resulting in pulmonary and systemic oxidative stress, had no effect on hepatic alpha-tocopherol concentrations or hepatic TTP. Overall, changes in hepatic TTP concentrations were minimal in response to dietary vitamin E levels or ETS-related oxidative stress. Thus, hepatic TTP concentrations may be at sufficient levels such that they are unaffected by either modulations of dietary vitamin E or by the conditions of environmentally related oxidative stress used in the present studies.

Alternate JournalLipids
PubMed ID17707975
Grant ListES011985 / ES / NIEHS NIH HHS / United States