TitleReactivation of outer-arm-depleted lung axonemes: evidence for functional differences between inner and outer dynein arms in situ.
Publication TypeJournal Article
Year of Publication1992
AuthorsHard, R, Blaustein, KL, Scarcello, L
JournalCell Motil Cytoskeleton
Volume21
Issue3
Pagination199-209
Date Published10/1992
ISSN0886-1544
KeywordsAdenosine Triphosphate, Animals, Cilia, Dyneins, Epithelial Cells, Epithelium, Hot Temperature, Hydrogen-Ion Concentration, Image Processing, Computer-Assisted, Lung, Microscopy, Models, Biological, Salamandridae, Salts, Substrate Specificity, Video Recording
Abstract
 

Demembranated axonemes isolated from newt lung ciliated cells show a complex beat frequency response to varying [MgATP] and temperature [Hard and Cypher, 1992, Cell Motil. Cytoskeleton 21:187-198]. The present study was undertaken to ascertain whether the beat frequency of outer-arm-depleted newt lung axonemes is controlled in a manner similar to that of intact axonemes. Populations of demembranated ciliary axonemes were isolated by Triton X-100 extraction of lungs from the newt, Taricha granulosa. Aliquots of the demembranated axonemes were further treated with solutions containing high salt (0.375 M KC1) and 1.25 mM MgATP. This treatment resulted in the selective removal of outer dynein arms and a concomitant decrease in beat frequency to a stable level, 33-35% of control values. The effects of pH, salt concentration, nucleotides, and temperature on the beat frequency of reactivated outer-arm-depleted axonemes were ascertained and compared with those of intact axonemes. Some reactivation properties, such as nucleotide specificity, the effect of pH on beat frequency and the threshold [MgATP] required for reactivation (approximately 5 microM) were similar to those observed for intact axonemes. Other properties, such as the relationship between beat frequency and varying [MgATP] or salt concentration, differed both qualitatively and quantitatively from those of control axonemes, as did their response to temperature over the range, 5 degrees-32 degrees C. The nature of the results obtained with temperature and MgATP suggests that inner and outer dynein arms are not functionally equivalent in situ.

DOI10.1002/cm.970210304
Alternate JournalCell Motil. Cytoskeleton
PubMed ID1533820
Grant ListGM21741 / GM / NIGMS NIH HHS / United States
HL29233 / HL / NHLBI NIH HHS / United States