TitleParadoxical effects of cycloheximide on the ultra-rapid induction of two hepatic mRNA sequences by triiodothyronine (T3).
Publication TypeJournal Article
Year of Publication1982
AuthorsSeelig, S, Jump, DB, Towle, HC, Liaw, C, Mariash, CN, Schwartz, HL, Oppenheimer, JH
Date Published1982 Feb
KeywordsAnimals, Base Sequence, Cycloheximide, Liver, Male, Protein Biosynthesis, Rats, Receptors, Cell Surface, Receptors, Thyroid Hormone, RNA, Messenger, Triiodothyronine


Triiodothyronine (T3) rapidly induces the accumulation of two hepatic mRNA sequences (spot 14 and spot CyT) in thyroidectomized rats as revealed by two-dimensional gel electrophoresis of in vitro translated products of isolated poly(A) containing RNA. T3 acting alone induced a 29-fold increase of spot 14 within 4 h, an increase which was completely inhibited by the concomitant administration of cycloheximide. On the other hand, CyT could be detected only after the administration of cycloheximide, and the combined action of cycloheximide and T3 resulted in a 4-fold increase in CyT 4 h after administration of both agents. These observations suggested that the early cellular action of T3 is contingent on the participation of rapidly turning over protein and mRNA.

Alternate JournalEndocrinology
PubMed ID6276146
Grant ListAM00800 / AM / NIADDK NIH HHS / United States
AM19812 / AM / NIADDK NIH HHS / United States
AM26919 / AM / NIADDK NIH HHS / United States