|Title||Ovarian cyst fluids are a cache of tumor biomarkers that include calgranulin A and calgranulin B isoforms.|
|Publication Type||Journal Article|
|Year of Publication||2013|
|Authors||Skaggs, HS, Saunders, BA, Miller, RW, Goodrich, ST, King, MS, Kimbler, KD, Branscum, AJ, Fung, ET, DePriest, PD, van Nagell, JR, Ueland, FR, Baron, AT|
|Keywords||Adult, Aged, Aged, 80 and over, Biomarkers, Tumor, Blotting, Western, Calgranulin A, Calgranulin B, Cyst Fluid, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay, Female, Humans, Middle Aged, Ovarian Cysts, Ovarian Neoplasms, Protein Isoforms, Sensitivity and Specificity, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization|
SELDI-TOF MS analysis of cyst fluids identified 95 peaks that discriminate malignant, borderline, and benign ovarian tumors. Three prominent peaks, which correspond to calgranulin A (m/z 10847) and two isoforms of calgranulin B (m/z 12717 and 13294), have higher concentrations in borderline and malignant cyst fluids. Together, calgranulin A and B distinguish borderline and malignant tumors from benign tumors with 28.6% and 63.6% sensitivity for early stage disease, respectively, at 95% specificity and with 74.8% accuracy. Ovarian cyst fluids are useful for discovering discriminatory biomarkers, such as calgranulin, which may have utility for detecting, diagnosing, and biochemically classifying ovarian tumors.
|Alternate Journal||Cancer Invest.|