TitleOvarian cyst fluids are a cache of tumor biomarkers that include calgranulin A and calgranulin B isoforms.
Publication TypeJournal Article
Year of Publication2013
AuthorsSkaggs, HS, Saunders, BA, Miller, RW, Goodrich, ST, King, MS, Kimbler, KD, Branscum, AJ, Fung, ET, DePriest, PD, van Nagell, JR, Ueland, FR, Baron, AT
JournalCancer Invest
Volume31
Issue7
Pagination433-53
Date Published08/2013
ISSN1532-4192
KeywordsAdult, Aged, Aged, 80 and over, Biomarkers, Tumor, Blotting, Western, Calgranulin A, Calgranulin B, Cyst Fluid, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay, Female, Humans, Middle Aged, Ovarian Cysts, Ovarian Neoplasms, Protein Isoforms, Sensitivity and Specificity, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Abstract

SELDI-TOF MS analysis of cyst fluids identified 95 peaks that discriminate malignant, borderline, and benign ovarian tumors. Three prominent peaks, which correspond to calgranulin A (m/z 10847) and two isoforms of calgranulin B (m/z 12717 and 13294), have higher concentrations in borderline and malignant cyst fluids. Together, calgranulin A and B distinguish borderline and malignant tumors from benign tumors with 28.6% and 63.6% sensitivity for early stage disease, respectively, at 95% specificity and with 74.8% accuracy. Ovarian cyst fluids are useful for discovering discriminatory biomarkers, such as calgranulin, which may have utility for detecting, diagnosing, and biochemically classifying ovarian tumors.
DOI10.3109/07357907.2013.802799
Alternate JournalCancer Invest.
PubMed ID23915068