Title | Ovarian cyst fluids are a cache of tumor biomarkers that include calgranulin A and calgranulin B isoforms. |
Publication Type | Journal Article |
Year of Publication | 2013 |
Authors | Skaggs, HS, Saunders, BA, Miller, RW, Goodrich, ST, King, MS, Kimbler, KD, Branscum, AJ, Fung, ET, DePriest, PD, van Nagell, JR, Ueland, FR, Baron, AT |
Journal | Cancer Invest |
Volume | 31 |
Issue | 7 |
Pagination | 433-53 |
Date Published | 08/2013 |
ISSN | 1532-4192 |
Keywords | Adult, Aged, Aged, 80 and over, Biomarkers, Tumor, Blotting, Western, Calgranulin A, Calgranulin B, Cyst Fluid, Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay, Female, Humans, Middle Aged, Ovarian Cysts, Ovarian Neoplasms, Protein Isoforms, Sensitivity and Specificity, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization |
Abstract | SELDI-TOF MS analysis of cyst fluids identified 95 peaks that discriminate malignant, borderline, and benign ovarian tumors. Three prominent peaks, which correspond to calgranulin A (m/z 10847) and two isoforms of calgranulin B (m/z 12717 and 13294), have higher concentrations in borderline and malignant cyst fluids. Together, calgranulin A and B distinguish borderline and malignant tumors from benign tumors with 28.6% and 63.6% sensitivity for early stage disease, respectively, at 95% specificity and with 74.8% accuracy. Ovarian cyst fluids are useful for discovering discriminatory biomarkers, such as calgranulin, which may have utility for detecting, diagnosing, and biochemically classifying ovarian tumors. |
DOI | 10.3109/07357907.2013.802799 |
Alternate Journal | Cancer Invest. |
PubMed ID | 23915068 |