|Title||Morphological and functional characteristics of skeletal muscle fibers from hormone-replaced and nonreplaced postmenopausal women.|
|Publication Type||Journal Article|
|Year of Publication||2003|
|Authors||Widrick, JJ, Maddalozzo, GF, Lewis, D, Valentine, BA, Garner, DP, Stelzer, JE, Shoepe, TC, Snow, CM|
|Journal||The journals of gerontology. Series A, Biological sciences and medical sciences|
|Date Published||2003 Jan|
|Keywords||Sensitivity and Specificity|
We tested the hypothesis that cross-bridge mechanisms of contraction differed in early postmenopausal women who did or did not receive hormone replacement therapy (HRT). Vastus lateralis biopsies were obtained from 17 postmenopausal women (49-57 years old), 8 of whom were on HRT for the previous 24 +/- 5 months and 9 of whom were never on HRT. Electrophoresis and enzyme histochemistry revealed that fiber myosin heavy chain (MHC) isoform distribution, the cross-sectional area (CSA) of slow and fast fibers, and the relative CSA occupied by each, were similar for HRT and non-HRT groups. Single permeabilized fibers containing type IIa MHC had greater Ca(2+)-activated peak specific force, unloaded shortening velocity, and peak power than fibers containing type I MHC, but in all cases the values for HRT and non-HRT groups were similar. In this cross-sectional study, we found no evidence that Ca(2+)-activated fiber function, MHC isoform distribution, or relative CSA occupied by slow and fast fibers differed between HRT and non-HRT groups.