Title | Morphological and functional characteristics of skeletal muscle fibers from hormone-replaced and nonreplaced postmenopausal women. |
Publication Type | Journal Article |
Year of Publication | 2003 |
Authors | Widrick, JJ, Maddalozzo, GF, Lewis, D, Valentine, BA, Garner, DP, Stelzer, JE, Shoepe, TC, Snow, CM |
Journal | The journals of gerontology. Series A, Biological sciences and medical sciences |
Volume | 58 |
Issue | 1 |
Pagination | 3-10 |
Date Published | 2003 Jan |
Keywords | Sensitivity and Specificity |
Abstract | We tested the hypothesis that cross-bridge mechanisms of contraction differed in early postmenopausal women who did or did not receive hormone replacement therapy (HRT). Vastus lateralis biopsies were obtained from 17 postmenopausal women (49-57 years old), 8 of whom were on HRT for the previous 24 +/- 5 months and 9 of whom were never on HRT. Electrophoresis and enzyme histochemistry revealed that fiber myosin heavy chain (MHC) isoform distribution, the cross-sectional area (CSA) of slow and fast fibers, and the relative CSA occupied by each, were similar for HRT and non-HRT groups. Single permeabilized fibers containing type IIa MHC had greater Ca(2+)-activated peak specific force, unloaded shortening velocity, and peak power than fibers containing type I MHC, but in all cases the values for HRT and non-HRT groups were similar. In this cross-sectional study, we found no evidence that Ca(2+)-activated fiber function, MHC isoform distribution, or relative CSA occupied by slow and fast fibers differed between HRT and non-HRT groups. |