TitleInfluence of omega-3 fatty acids on skeletal muscle protein metabolism and mitochondrial bioenergetics in older adults.
Publication TypeJournal Article
Year of Publication2017
AuthorsLalia, AZ, Dasari, S, Robinson, MM, Abid, H, Morse, DM, Klaus, KA, Lanza, IR
JournalAging (Albany NY)
Volume9
Issue4
Pagination1096-1129
Date Published2017 Apr
ISSN1945-4589
Abstract
 

Omega-3 polyunsaturated fatty acids (n3-PUFA) are recognized for their anti-inflammatory effects and may be beneficial in the context of sarcopenia. We determined the influence of n3-PUFA on muscle mitochondrial physiology and protein metabolism in older adults. Twelve young (18-35 years) and older (65-85 years) men and women were studied at baseline. Older adults were studied again following n3-PUFA supplementation (3.9g/day, 16 weeks). Muscle biopsies were used to evaluate respiratory capacity (high resolution respirometry) and oxidant emissions (spectrofluorometry) in isolated mitochondria. Maximal respiration was significantly lower in older compared to young. n3-PUFA did not change respiration, but significantly reduced oxidant emissions. Participants performed a single bout of resistance exercise, followed by biopsies at 15 and 18 hours post exercise. Several genes involved in muscle protein turnover were significantly altered in older adults at baseline and following exercise, yet muscle protein synthesis was similar between age groups under both conditions. Following n3-PUFA supplementation, mixed muscle, mitochondrial, and sarcoplasmic protein synthesis rates were increased in older adults before exercise. n3-PUFA increased post-exercise mitochondrial and myofibrillar protein synthesis in older adults. These results demonstrate that n3-PUFA reduce mitochondrial oxidant emissions, increase postabsorptive muscle protein synthesis, and enhance anabolic responses to exercise in older adults.

DOI10.18632/aging.101210
Alternate JournalAging (Albany NY)
PubMed ID28379838
PubMed Central IDPMC5425117
Grant ListKL2 TR000136 / TR / NCATS NIH HHS / United States
U24 DK100469 / DK / NIDDK NIH HHS / United States
UL1 TR000135 / TR / NCATS NIH HHS / United States