TitleBone Marrow Adipose Tissue Deficiency Increases Disuse-Induced Bone Loss in Male Mice.
Publication TypeJournal Article
Year of Publication2017
AuthorsKeune, JA, Wong, CP, Branscum, AJ, Iwaniec, UT, Turner, RT
JournalSci Rep
Volume7
Pagination46325
Date Published04/2017
ISSN2045-2322
KeywordsAdipose Tissue, Alveolar Bone Loss, Animals, Biomechanical Phenomena, Bone Density, Bone Marrow, Disease Models, Animal, Gene Expression Profiling, Genotype, Hindlimb Suspension, Male, Mice, Mice, Knockout, Osteoblasts, Osteoclasts, Transcriptome, X-Ray Microtomography
Abstract
 

Bone marrow adipose tissue (MAT) is negatively associated with bone mass. Since osteoblasts and adipocytes are derived from the same precursor cells, adipocyte differentiation may occur at the expense of osteoblast differentiation. We used MAT-deficient Kit (MAT-) mice to determine if absence of MAT reduced bone loss in hindlimb-unloaded (HU) mice. Male MAT- and wild-type (WT) mice were randomly assigned to a baseline, control or HU group (n = 10 mice/group) within each genotype and HU groups unloaded for 2 weeks. Femurs were evaluated using micro-computed tomography, histomorphometry and targeted gene profiling. MAT- mice had a greater reduction in bone volume fraction after HU than did WT mice. HU MAT- mice had elevated cancellous bone formation and resorption compared to other treatment groups as well as a unique profile of differentially expressed genes. Adoptive transfer of WT bone marrow-derived hematopoietic stem cells reconstituted c-kit but not MAT in Kit mice. The MAT- WT → Kit mice lost cancellous bone following 2 weeks of HU. In summary, results from this study suggest that MAT deficiency was not protective, and was associated with exaggerated disuse-induced cancellous bone loss.

DOI10.1038/srep46325
Alternate JournalSci Rep
PubMed ID28402337
PubMed Central IDPMC5389344