TitleAntenatal Influenza A-Specific IgA, IgM, and IgG Antibodies in Mother's Own Breast Milk and Donor Breast Milk, and Gastric Contents and Stools from Preterm Infants.
Publication TypeJournal Article
Year of Publication2019
AuthorsDemers-Mathieu, V, Huston, RK, Markell, AM, McCulley, EA, Martin, RL, Dallas, DC
JournalNutrients
Volume11
Issue7
Date Published07/2019
ISSN2072-6643
KeywordsAntibodies, Viral, Feces, Female, Gastrointestinal Contents, Humans, Immunity, Maternally-Acquired, Immunoglobulin A, Immunoglobulin G, Immunoglobulin M, Infant, Premature, Influenza A Virus, H1N1 Subtype, Influenza A Virus, H3N2 Subtype, Milk, Human, Mothers
Abstract
 

Antenatal milk anti-influenza antibodies may provide additional protection to newborns until they are able to produce their own antibodies. To evaluate the relative abundance of milk, we studied the antibodies specific to influenza A in feeds and gastric contents and stools from preterm infants fed mother's own breast milk (MBM) and donor breast milk (DBM). Feed (MBM or DBM) and gastric contents (MBM or DBM at 1 h post-ingestion) and stool samples (MBM/DBM at 24 h post-ingestion) were collected, respectively, from 20 preterm (26-36 weeks gestational age) mother-infant pairs at 8-9 days and 21-22 days of postnatal age. Samples were analyzed via ELISA for anti-H1N1 hemagglutinin (anti-H1N1 HA) and anti-H3N2 neuraminidase (anti-H3N2 NA) specificity across immunoglobulin A (IgA), immunoglobulin M (IgM), and immunoglobulin G (IgG) isotypes. The relative abundance of influenza A-specific IgA in feeds and gastric contents were higher in MBM than DBM at 8-9 days of postnatal age but did not differ at 21-22 days. Anti-influenza A-specific IgM was higher in MBM than in DBM at both postnatal times in feed and gastric samples. At both postnatal times, anti-influenza A-specific IgG was higher in MBM than DBM but did not differ in gastric contents. Gastric digestion reduced anti-H3N2 NA IgG from MBM at 21-22 days and from DBM at 8-9 days of lactation, whereas other anti-influenza A antibodies were not digested at either postnatal times. Supplementation of anti-influenza A-specific antibodies in DBM may help reduce the risk of influenza virus infection. However, the effective antibody dose required to induce a significant protective effect remains unknown.

DOI10.3390/nu11071567
Alternate JournalNutrients
PubMed ID31336756
PubMed Central IDPMC6682892
Grant ListR00HD079561 / / Eunice Kennedy Shriver National Institute of Child Health and Human Development /
2017-1586 / / Gerber Foundation /