TitleAcute {beta}-adrenergic stimulation does not alter mitochondrial protein synthesis or markers of mitochondrial biogenesis in adult men.
Publication TypeJournal Article
Year of Publication2010
AuthorsRobinson, MM, Richards, JC, Hickey, MS, Moore, DR, Phillips, SM, Bell, C, Miller, BF
JournalAm J Physiol Regul Integr Comp Physiol
Date Published2010 Jan
KeywordsAdrenergic beta-Agonists, Biopsy, Blood Pressure, Heart Rate, Heat-Shock Proteins, Humans, Isoproterenol, Male, Mitochondria, Muscle, Mitochondrial Proteins, Muscle Proteins, Muscle, Skeletal, Myofibrils, Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha, Receptors, Adrenergic, beta, Transcription Factors, Young Adult

Exercise-induced expression of peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC-1alpha) is dramatically inhibited in mice pretreated with a beta-adrenergic receptor (beta-AR) antagonist, suggesting that beta-ARs play an important role in the regulation of skeletal muscle PGC-1alpha expression, and potentially, mitochondrial biogenesis. Accordingly, we hypothesized that acute beta-AR stimulation would induce transcriptional pathways involved in skeletal muscle mitochondrial biogenesis in humans. Whole body protein turnover (WBPT), myofibrillar protein synthesis (MyPS), skeletal muscle mitochondrial protein synthesis (MiPS), and mitochondrial biogenic signaling were determined in samples of vastus lateralis obtained on two separate occasions in 10 young adult males following 1 h of continuous intravenous administration of saline (CON) or a nonspecific beta-AR agonist [isoproterenol (ISO): 12 ng.kg fat free mass(-1).min(-1)], combined with coinfusion of [1,2](13)C-leucine. beta-AR stimulation induced appreciable increases in heart rate and systolic blood pressure (both P < 0.001) but did not affect mitochondrial biogenic signaling (no change in PGC-1alpha, TFAM, NRF-1, NRF-2, COX, or NADHox expression via RT-PCR; P > 0.05). Additionally, MiPS [CON: 0.099 +/- 0.028, ISO: 0.074 +/- 0.046 (mean +/- SD); P > 0.05] and MyPS (CON: 0.059 +/- 0.008, ISO: 0.055 +/- 0.009; P > 0.05), as well as measures of WBPT were unaffected. On the basis of this investigation, we conclude that acute intravenous beta-AR stimulation does not increase mitochondrial protein synthesis or biogenesis signals in skeletal muscle.

Alternate JournalAm. J. Physiol. Regul. Integr. Comp. Physiol.
PubMed ID19907002